I just realized you wrote: "I flashed it using DeFuse."
It was supposed to be flashed with the correct firmware version.
What firmware version did you flash ?
New PCB (1.6-100) only works with firmware 1.09 and newer.
As written in firmware 1.09 : "With these new PCBs, the knobs and butons won't respond with earlier firmware."
Please flash latest version :
https://github.com/Ixox/preenfm3/releaseshttps://github.com/Ixox/preenfm3/wiki/Upgrade-firmware-(Flash-bootloader)
The pfm3 bootloader won't recognize the controlboard neither.
You'll need to flash it too.
Misc info :
The led on the control baord uses a different pins from the button/encoders.
Boot/DFU jumper on boots to System (STM32) bootloader.
Pressing the menu buton enteres the preenfm3 bootloader (which looks different from the firmware). It's written boot loader at the top.
SDCard error is something different, connection to the SD Card is the 4 top pins of the display.
This cannot be link to the fact that you press the MENU button.
Make sure the SD card is formated, contais the pfm3/ folder and is well inserted.